| Western blot (WB): | 1:2000 |
| Immunohistochemistry (IHC): | 1:150 |

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-NEGR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M06730) (1:150).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEGR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEGR1 (Cat# M06730)(1:2000).

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-NEGR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M06730) (1:150).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEGR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEGR1 (Cat# M06730)(1:2000).

