Anti-PRKAR1B Antibody (Clone#OTI2A3)

Mouse monoclonal antibody

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M07227

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKAR1B antibody, and then analyzed by flow cytometry.
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M07227)
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Flow cytometric Analysis of Hela cells, using anti-PRKAR1B antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M07227)
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Anti-PRKAR1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1B .
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Flow cytometric Analysis of Jurkat cells, using anti-PRKAR1B antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1B (Cat# M07227).
all(7)

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产品简介>

产品名称

Anti-PRKAR1B Antibody (Clone#OTI2A3)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

PRKAR1; PRKAR1B

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

PRKAR1B

克隆号

OTI2A3

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human PRKAR1B (NP_002726) produced in HEK293T cell.

计算分子量

43 kDa

实际分子量

42.9 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP. [supplied by OMIM, Jun 2009]

Uniprot ID

P31321

文献引用格式

PRKAR1B Antibody (Clone#OTI2A3) (Boster Biological Technology, Wuhan, China. Catalog#M07227)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cAMP-dependent protein kinase type I-beta regulatory subunit

实验方案>

实验步骤

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