Western blot (WB): | 1:500~2000 |
Immunohistochemistry (IHC): | 1:500 |
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and ABCF2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 monoclonal antibody M10873, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)
Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-ABCF2 monoclonal antibody (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 (Cat# M10873)(1:2000).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and ABCF2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 monoclonal antibody M10873, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)
Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-ABCF2 monoclonal antibody (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 (Cat# M10873)(1:2000).