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Anti-ABCF2 Antibody (Clone#OTI8F4)

Mouse monoclonal antibody

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筛选器: All WB IHC

M10873

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and ABCF2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 monoclonal antibody M10873, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control.

    all(6)
  • Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)

    all(6)
  • Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)

    all(6)
  • Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)

    all(6)
  • Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-ABCF2 monoclonal antibody (1:500).

    all(6)
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 (Cat# M10873)(1:2000).

    all(6)

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产品名称
Anti-ABCF2 Antibody (Clone#OTI8F4)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ABC28; ABCF2; EST133090; HUSSY 18; HUSSY18; M ABC1
产品类型
Monoclonal
检验物种
human, mouse
应用范围
WB, IHC
基因名称
ABCF2
克隆号
OTI8F4
宿主
Mouse
抗体亚型
IgG1
免疫原
Human recombinant protein fragment corresponding to amino acids 1-235 of human ABCF2 (NP_009120) produced in E.coli.
计算分子量
71 kDa
实际分子量
71.1 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Related pseudogenes have been identified on chromosomes 3 and 7.
Uniprot ID
Q9UG63  
文献引用格式
ABCF2 Antibody (Clone#OTI8F4) (Boster Biological Technology, Wuhan, China. Catalog#M10873)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
ATP-binding cassette sub-family F member 2
推荐稀释比
Western blot (WB):1:500~2000
Immunohistochemistry (IHC):1:500

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    Anti-ABCF2 Antibody (Clone#OTI8F4)

    筛选器: All WB IHC

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