Anti-ATPB/ATP5F1B Antibody (Clone#OTI1F7)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

M32270

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M32270, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M32270, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M32270, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ATP5 Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M32270, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M32270, Dilution 1:50)
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Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.
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Anti-ATP5B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B .
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HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal (M32270), and then analyzed by flow cytometry.
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Flow cytometric analysis of Hela cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ATPB/ATP5F1B Antibody (Clone#OTI1F7)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ATP5B; ATPB; ATPMB; ATPSB; Cell and organelle markers; Mitochondria Marker; Mitochondrial Marker; Mitochondrion Marker

产品类型

Monoclonal

检验物种

human, dog, monkey

应用范围

WB, IHC, ICC/IF, FCM

基因名称

ATP5F1B

克隆号

OTI1F7

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.

计算分子量

57 kDa

实际分子量

56.6 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Uniprot ID

P06576

文献引用格式

ATPB/ATP5F1B Antibody (Clone#OTI1F7) (Boster Biological Technology, Wuhan, China. Catalog#M32270)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ATP synthase F1 subunit beta

蛋白名全称

ATP synthase subunit beta, mitochondrial

实验方案>

实验步骤

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