| Western blot (WB): | 1:4000 |
| Immunohistochemistry (IHC): | 1:150 |

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-SNCA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00215) (1:500)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNCA mouse monoclonal antibody. (MA00215) (1:150).

Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-SNCA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00215) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNCA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNCA (Cat# MA00215).

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-SNCA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00215) (1:500)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNCA mouse monoclonal antibody. (MA00215) (1:150).

Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-SNCA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00215) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNCA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNCA (Cat# MA00215).



