Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and KEAP1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody MA00514. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control (1:200).
all(4)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then analyzed by flow cytometry.
all(4) 
Anti-KEAP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KEAP1 .
all(4) 
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).
all(4) | Western blot (WB): | 1:2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
| Flow cytometry (FCM): | 1:100 |
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and KEAP1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody MA00514. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control (1:200).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then analyzed by flow cytometry.
Anti-KEAP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KEAP1 .
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and KEAP1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody MA00514. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control (1:200).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then analyzed by flow cytometry.
Anti-KEAP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KEAP1 .
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).
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