Anti-HSP70 Antibody (Clone#OTI2C12)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

MA00949

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-HSP70 antibody (MA00949). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse kidney whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-HSP70 antigen affinity purified monoclonal antibody (MA00949) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSP70 mouse monoclonal antibody (MA00949). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00949, Dilution 1:50
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-HSP70 mouse monoclonal antibody (MA00949). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00949, Dilution 1:50)
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Immunofluorescent staining of HepG2 cells using anti-HSP70 mouse monoclonal antibody (MA00949) (1:50).
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Anti-HSP70 mouse monoclonal antibody (MA00949) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSP70 (1:100).
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Flow cytometric analysis of permeabilized Hela cells, using anti-HSP70 antibody, compared to an isotype control (green), and a PBS control (blue) (1:100).
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Flow cytometric analysis of permeabilized Jurkat cells, using anti-HSP70 antibody (MA00949), compared to an isotype control (green), and a PBS control (blue) (1:100).
all(7)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-HSP70 Antibody (Clone#OTI2C12)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HSP70-1; HSP70.1; HSPA1A; HSP72; HSPA1; HSX70; HEL-S-103; HSP70; HSP70-1A; HSP70-2; HSP70.2; HSP70I; HSP72; HSPA1

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, IHC, ICC/IF, FCM

基因名称

HSPA1A

克隆号

OTI2C12

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.

计算分子量

70 kDa

实际分子量

70 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Uniprot ID

P0DMV8

文献引用格式

HSP70 Antibody (Clone#OTI2C12) (Boster Biological Technology, Wuhan, China. Catalog#MA00949)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

heat shock protein family A (Hsp70) member 1A

蛋白名全称

Heat shock 70 kDa protein 1A?

实验方案>

实验步骤

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