Anti-HSP70 Antibody (Clone#DGE-8)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4335

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-HSP70 antibody (BM4335). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP70 antigen affinity purified monoclonal antibody (BM4335) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.
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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using HSP70 Antibody.
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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using HSP70 Antibody.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using HSP70 Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast, using HSP70 Antibody.
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Immunofluorescent analysis of Hela cells, using HSP70 Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-HSP70 Antibody (Clone#DGE-8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HSP70-1; HSP70.1; HSPA1A; HSP72; HSPA1; HSX70; HEL-S-103; HSP70; HSP70-1A; HSP70-2; HSP70.2; HSP70I; HSP72; HSPA1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

HSPA1A

克隆号

DGE-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Hsp70

计算分子量

70 kDa

实际分子量

70 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

HSPA1(heat shock 70kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al.(1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

Uniprot ID

P0DMV8

文献引用格式

HSP70 Antibody (Clone#DGE-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4335)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Heat shock 70 kDa protein 1A

实验方案>

实验步骤

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