Anti-PRKG1 Antibody (Clone#OTI9G4)

Mouse monoclonal antibody

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MA01708

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PRKG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01708, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PRKG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01708, Dilution 1:50)
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Anti-PRKG1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKG1 .
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG1(Cat# MA01708).
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产品简介>

产品名称

Anti-PRKG1 Antibody (Clone#OTI9G4)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

cGK 1; cGK1; CGKI; cGKI alpha; cGKI BETA; PGK; PKG; PRKG1; PRKG1B; PRKGR1A; PRKGR1B; Protein Kinase G1 alpha

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

PRKG1

克隆号

OTI9G4

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human PRKG1 (NP_006249) produced in HEK293T cell.

计算分子量

76 kDa

实际分子量

78 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

cGMP-dependent protein kinase 1, alpha isozyme is an enzyme that in humans is encoded by the PRKG1 gene. Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

Uniprot ID

Q13976

文献引用格式

PRKG1 Antibody (Clone#OTI9G4) (Boster Biological Technology, Wuhan, China. Catalog#MA01708)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

protein kinase cGMP-dependent 1

蛋白名全称

cGMP-dependent protein kinase 1

实验方案>

实验步骤

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