Anti-CHEK2 (Phospho-T68) Antibody (Clone#27C74)

50μl|1280 100μl|2180 150μl|2800

CDS1; CHEK2; Chk2; HuCds1; LFS2; PP1425; RAD53

Monoclonal

human

WB, IP

CHEK2

27C74

Rabbit

IgG

A synthesized peptide derived from human Phospho-Chk2 (T68)

61 kDa

61 kDa

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

Affinity-chromatography

500 ug/ml

Liquid

12 months from date of receipt，-20℃ as supplied.

CHK2, a protein kinase that is activated in response to DNA damage, is involved in cell cycle arrest. Mapped on 22q12.1, CHK2 has a potential regulatory region rich in SQ and TQ amino acid pairs. It regulates BRCA1 function after DNA damage by phosphorylating serine-988 of BRCA1. Additionally, CHK2 can be modified by phosphorylation and activated in response to ionizing radiation, and can be also modified in response to hydroxyurea treatment. Furthermore, oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells. Moreover, CHK2 is a tumor suppressor gene conferring predisposition to sarcoma, breast cancer, and brain tumors, and that their observations provided a link between the central role of p53 inactivation in human cancer and the well-defined G2 checkpoint in yeast. There is a wide expression of small amounts of CHK2 mRNA with larger amounts in human testis, spleen, colon, and peripheral blood leukocytes.

O96017  

CHEK2 (Phospho-T68) Antibody (Clone#27C74) (Boster Biological Technology, Wuhan, China. Catalog#P00277-1)

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

checkpoint kinase 2

Western blot (WB):	1:500-2000
ImmunoPrecipitation (IP):	1:50