Anti-Beta Catenin/CTNNB1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PA1212

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Beta Catenin/CTNNB1 using anti-Beta Catenin/CTNNB1 antibody (PA1212). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MM453 whole cell lysates ,
Lane 2: MCF-7 whole cell lysates ,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Catenin/CTNNB1 antigen affinity purified polyclonal antibody (PA1212) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Catenin/CTNNB1 at approximately 95 kDa. The expected band size for Beta Catenin/CTNNB1 is at 85 kDa.
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IHC analysis of Beta Catenin/CTNNB1 using anti-Beta Catenin/CTNNB1 antibody (PA1212).
Beta Catenin/CTNNB1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-Beta Catenin/CTNNB1 Antibody (PA1212) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Beta Catenin/CTNNB1 using anti-Beta Catenin/CTNNB1 antibody (PA1212).
Beta Catenin/CTNNB1 was detected in frozen section of rat intestine tissue. The tissue section was incubated with rabbit anti-Beta Catenin/CTNNB1 Antibody (PA1212) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of Beta Catenin/CTNNB1 using anti-Beta Catenin/CTNNB1 antibody (PA1212).
Beta Catenin/CTNNB1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-Beta Catenin/CTNNB1 Antibody (PA1212) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

Anti-Beta Catenin/CTNNB1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

b cat; Beta catenin; beta-Catenin; Catenin beta 1; CTNNB; CTNNB1; PRO2286; β catenin; β-catenin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

CTNNB1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human beta Catenin, identical to the related rat and mouse sequences.

计算分子量

85 kDa

实际分子量

95 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development(both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Uniprot ID

P35222

RRID

AB_3082600

文献引用格式

Beta Catenin/CTNNB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA1212)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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