Anti-DLD Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PA1463

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of DLD using anti-DLD antibody (PA1463). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DLD antigen affinity purified polyclonal antibody (PA1463) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DLD at approximately 54 kDa. The expected band size for DLD is at 54 kDa.
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Western blot analysis of DLD using anti-DLD antibody (PA1463). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Siha whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DLD antigen affinity purified polyclonal antibody (PA1463) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150). A specific band was detected for DLD at approximately 54 kDa. The expected band size for DLD is at 54 kDa.
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IHC analysis of DLD using anti-DLD antibody (PA1463).
DLD was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-DLD Antibody (PA1463) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of DLD using anti-DLD antibody (PA1463).
DLD was detected in frozen section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-DLD Antibody (PA1463) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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ICC analysis of DLD using anti- DLD antibody (PA1463).
DLD was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-DLD Antibody (PA1463) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of DLD using anti-DLD antibody (PA1463).
DLD was detected in an immunocytochemical section of HCT116 cells. The section was incubated with rabbit anti-DLD Antibody (PA1463) at a dilution of 1:100. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)(Catalog#BA1105) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-DLD Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

dihydrolipoamide dehydrogenase; DLD; DLDH; E3; GCSL; LAD; PHE3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

DLD

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human DLD, different from the related mouse and rat sequences by one amino acid.

计算分子量

54 kDa

实际分子量

54 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex(BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Uniprot ID

P09622

文献引用格式

DLD Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA1463)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

dihydrolipoamide dehydrogenase

蛋白名全称

Dihydrolipoyl dehydrogenase, mitochondrial

实验方案>

实验步骤

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