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Anti-DLD Antibody (Clone#OTI6D5)

Mouse monoclonal antibody

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筛选器: All WB IHC ICC/IF FCM

M00870

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and DLD-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-DLD monoclonal antibody M00870 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  • Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DLD monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  • Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-DLD monoclonal antibody (1:200).

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  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD(Cat# M00870).

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  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)

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  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)

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  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)

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  • Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)

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  • Anti-DLD mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD .

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  • HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DLD antibody, and then analyzed by flow cytometry.

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产品名称
Anti-DLD Antibody (Clone#OTI6D5)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
dihydrolipoamide dehydrogenase; DLD; DLDH; E3; GCSL; LAD; PHE3
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
DLD
克隆号
OTI6D5
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human DLD(NP_000099) produced in HEK293T cell.
计算分子量
54 kDa
实际分子量
50.1 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq]
Uniprot ID
P09622  
文献引用格式
DLD Antibody (Clone#OTI6D5) (Boster Biological Technology, Wuhan, China. Catalog#M00870)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Dihydrolipoyl dehydrogenase, mitochondrial|EC 1.8.1.4|Dihydrolipoamide dehydrogenase|Glycine cleavage system L protein
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:150
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100
Flow cytometry (FCM):1:100

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    Anti-DLD Antibody (Clone#OTI6D5)

    筛选器: All WB IHC ICC/IF FCM

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