Western blot (WB): | 1:2000 |
Immunohistochemistry (IHC): | 1:150 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
Flow cytometry (FCM): | 1:100 |
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and DLD-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-DLD monoclonal antibody M00870 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DLD monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-DLD monoclonal antibody (1:200).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD(Cat# M00870).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)
Anti-DLD mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD .
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DLD antibody, and then analyzed by flow cytometry.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and DLD-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-DLD monoclonal antibody M00870 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DLD monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-DLD monoclonal antibody (1:200).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD(Cat# M00870).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00870)
Anti-DLD mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD .
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DLD antibody, and then analyzed by flow cytometry.