Anti-FGF21 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PA1673

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of FGF21 using anti-FGF21 antibody (PA1673). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: A431 whole cell lysates,
Lane 3: HEPA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (PA1673) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF21 at approximately 22 kDa. The expected band size for FGF21 is at 22 kDa.
all(2)
Western blot analysis of FGF21 using anti-FGF21 antibody (PA1673). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (PA1673) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF21 at approximately 22 kDa. The expected band size for FGF21 is at 22 kDa.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-FGF21 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

FGF 21; FGF21; FGF-21; fibroblast growth factor 21

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

FGF21

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human FGF21, different from the related rat and mouse sequences by two amino acids.

计算分子量

22 kDa

实际分子量

22 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

FGF21(Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor(FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.

Uniprot ID

Q9NSA1

RRID

AB_3082753

文献引用格式

FGF21 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA1673)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

fibroblast growth factor 21

蛋白名全称

Fibroblast growth factor 21(FGF-21)

实验方案>

实验步骤

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