Anti-AFP Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB0090

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-AFP antibody (PB0090). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AFP antigen affinity purified polyclonal antibody (PB0090) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AFP at approximately 69 kDa. The expected band size for AFP is at 69 kDa.
all(2)
IHC analysis of AFP using anti-AFP antibody (PB0090).
AFP was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AFP Antibody (PB0090) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-AFP Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AFP; Alpha 1 fetoprotein; Alpha fetoglobulin; alpha fetoprotein; FETA; HPAFP

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

AFP

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.

计算分子量

69 kDa

实际分子量

69 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.

Uniprot ID

P02771

文献引用格式

AFP Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0090)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

alpha-fetoprotein

蛋白名全称

Alpha-fetoprotein

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实验步骤

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