Anti-AFP Antibody (Clone#OTI2F11)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF

M00522-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-AFP antibody (M00522-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AFP antigen affinity purified monoclonal antibody (M00522-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AFP at approximately 69 kDa. The expected band size for AFP is at 69 kDa.
all(5)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M00522-4) (1:150)
all(5)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M00522-4) (1:150)
all(5)
Immunofluorescent staining of A549 cells using anti-AFP mouse monoclonal antibody (1:100).
all(5)
Anti-AFP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP (1:100).
all(5)

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产品简介>

产品名称

Anti-AFP Antibody (Clone#OTI2F11)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AFP; Alpha 1 fetoprotein; Alpha fetoglobulin; alpha fetoprotein; FETA; HPAFP

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF

基因名称

AFP

克隆号

OTI2F11

宿主

Mouse

抗体亚型

IgG2b

免疫原

Human recombinant protein fragment corresponding to amino acids 19-397 of human AFP (NP_001125) produced in E.coli.

计算分子量

69 kDa

实际分子量

69 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Uniprot ID

P02771

文献引用格式

AFP Antibody (Clone#OTI2F11) (Boster Biological Technology, Wuhan, China. Catalog#M00522-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

alpha fetoprotein

蛋白名全称

Alpha-fetoprotein

实验方案>

实验步骤

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