| Western blot (WB): | 1:500-2000 |

Western blot analysis of GLUR4/GRIA4 using anti-GLUR4/GRIA4 antibody (PB0204). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLUR4/GRIA4 antigen affinity purified polyclonal antibody (PB0204) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLUR4/GRIA4 at approximately 101 kDa. The expected band size for GLUR4/GRIA4 is at 101 kDa.

Western blot analysis of GLUR4/GRIA4 using anti-GLUR4/GRIA4 antibody (PB0204). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLUR4/GRIA4 antigen affinity purified polyclonal antibody (PB0204) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLUR4/GRIA4 at approximately 101 kDa. The expected band size for GLUR4/GRIA4 is at 101 kDa.
