Anti-GLUR4/GRIA4 Antibody

筛选器： All WB FCM

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  Western blot analysis of GLUR4/GRIA4 using anti-GLUR4/GRIA4 antibody (PB0204). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Rat Brain tissue lysates,
  Lane 2: Mouse Brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLUR4/GRIA4 antigen affinity purified polyclonal antibody (PB0204) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLUR4/GRIA4 at approximately 101 kDa. The expected band size for GLUR4/GRIA4 is at 101 kDa.

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  Flow Cytometry analysis of A549 cells using anti- GRIA4 antibody (PB0204).
  Overlay histogram showing A549 cells stained with PB0204 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIA4 Antibody (PB0204, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Western blot analysis of GLUR4/GRIA4 using anti-GLUR4/GRIA4 antibody (PB0204). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Rat Brain tissue lysates,
  Lane 2: Mouse Brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLUR4/GRIA4 antigen affinity purified polyclonal antibody (PB0204) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLUR4/GRIA4 at approximately 101 kDa. The expected band size for GLUR4/GRIA4 is at 101 kDa.

• 

  Flow Cytometry analysis of A549 cells using anti- GRIA4 antibody (PB0204).
  Overlay histogram showing A549 cells stained with PB0204 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIA4 Antibody (PB0204, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.