Anti-SEMA3A Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0370

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SEMA3A using anti-SEMA3A antibody (PB0370). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SEMA3A antigen affinity purified polyclonal antibody (PB0370) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SEMA3A at approximately 89 kDa. The expected band size for SEMA3A is at 89 kDa.
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产品名称

Anti-SEMA3A Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

coll 1; Hsema I; Hsema III; Sema III; SEMA1; SEMA3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; Semaphorin III; SemD

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

SEMA3A

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Semaphorin 3A recombinant protein (Position: K550-V771). Human Semaphorin 3A shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Semaphorin 3A, respectively.

计算分子量

89 kDa

实际分子量

89 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SEMA3A is also known as COLL1, SEMA1 or SEMAL. This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.

Uniprot ID

Q14563

文献引用格式

SEMA3A Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0370)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

蛋白名全称

Semaphorin-3A

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