Anti-SMAD3 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PB0445

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SMAD3 using anti-SMAD3 antibody (PB0445). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat ovary tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD3 antigen affinity purified polyclonal antibody (PB0445) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD3 at approximately 50-55 kDa. The expected band size for SMAD3 is at 46 kDa.
all(3)
IHC analysis of SMAD3 using anti-SMAD3 antibody (PB0445).
SMAD3 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SMAD3 Antibody (PB0445) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
ICC/IF analysis of SMAD3 using anti-SMAD3 antibody (PB0445) and anti-Alpha Tubulin antibody (M03989-3).
SMAD3 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SMAD3 Antibody (PB0445) at a dilution of 1:100. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) (Catalog # BA1105) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
all(3)

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产品简介>

产品名称

Anti-SMAD3 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

hMAD 3; hSMAD3; HSPC193; HsT17436; JV15 2; MAD homolog 3; Mad3; MADH3; Mothers against DPP homolog 3; SMAD 3; SMAD family member 3; SMAD3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

SMAD3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Smad3 identical to the related mouse and rat sequences.

计算分子量

46 kDa

实际分子量

50-55 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Mothers against decapentaplegic homolog 3 also known as SMAD family member 3 or SMAD3 is a protein that in humans is encoded by the SMAD3 gene. SMAD3 is a member of the SMAD family of proteins. It is located in 15q22.33. And it mediates the signals from the transforming growth factor beta (TGF-β) super family ligands that regulate cell proliferation, differentiation and death. Based on its essential role in TGF beta signaling pathway, SMAD3 has been related with tumor growth in cancer development.

Uniprot ID

P84022

文献引用格式

SMAD3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0445)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SMAD family member 3

蛋白名全称

Mothers against decapentaplegic homolog 3

实验方案>

实验步骤

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