Anti-Thrombospondin 1/THBS1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0471

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Thrombospondin 1/THBS1 using anti-Thrombospondin 1/THBS1 antibody (PB0471). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Thrombospondin 1/THBS1 antigen affinity purified polyclonal antibody (PB0471) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Thrombospondin 1/THBS1 at approximately 170-180 kDa. The expected band size for Thrombospondin 1/THBS1 is at 129 kDa.
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产品名称

Anti-Thrombospondin 1/THBS1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

THBS; THBS 1; THBS1; thrombospondin 1; Thrombospondin-1; TSP; TSP 1; TSP1; TSP-1

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

THBS1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human THBS1 different from the related mouse sequence by five amino acids.

计算分子量

129 kDa

实际分子量

129 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

THBS1 is also known as Thrombospondin 1, TSP1. The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. It is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Also this protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. In addition, the thrombospondin-1 protein is a member of the thrombospondinfamily. It is a multi-domain matrix glycoprotein that has been shown to be a natural inhibitor of neovascularization and tumorigenesis in healthy tissue. Both positive and negative modulation of endothelial cell adhesion, motility, and growth have been attributed to TSP1. This should not be surprising considering that TSP1 interacts with at least 12 cell adhesion receptors, including CD36, αv integrins, β1 integrins, syndecan, and integrin-associated protein (IAP or CD47). It also interacts with numerous proteases involved in angiogenesis, including plasminogen, urokinase, matrix metalloproteinase, thrombin, cathepsin, and elastase.

Uniprot ID

P07996

文献引用格式

Thrombospondin 1/THBS1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0471)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

thrombospondin 1

蛋白名全称

Thrombospondin-1

实验方案>

实验步骤

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