Anti-MMP9 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

PB0709

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of MMP9 using anti-MMP9 antibody (PB0709). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MMP9 antigen affinity purified polyclonal antibody (PB0709) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MMP9 at approximately 78 kDa. The expected band size for MMP9 is at 79 kDa.
all(7)
IHC analysis of MMP9 using anti-MMP9 antibody (PB0709).
MMP9 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MMP9 Antibody (PB0709) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of MMP9 using anti-MMP9 antibody (PB0709).
MMP9 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MMP9 Antibody (PB0709) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of MMP9 using anti-MMP9 antibody (PB0709).
MMP9 was detected in a paraffin-embedded section of human lymphadenoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MMP9 Antibody (PB0709) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of MMP9 using anti-MMP9 antibody (PB0709).
MMP9 was detected in a paraffin-embedded section of human Lymph nodes of gastric adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MMP9 Antibody (PB0709) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of MMP9 using anti-MMP9 antibody (PB0709).
MMP9 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MMP9 Antibody (PB0709) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
all(7)

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产品简介>

产品名称

Anti-MMP9 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; Matrix metalloproteinase 9; MMP 9; MMP9; MMP-9; MMP9 (N-terminal)

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ELISA

基因名称

MMP9

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of rat MMP-9 different from the related human sequence by nineteen amino acids, and from the related mouse sequence by nine amino acids.

计算分子量

79 kDa

实际分子量

78 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Uniprot ID

P50282

文献引用格式

MMP9 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0709)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

蛋白名全称

Matrix metalloproteinase-9

实验方案>

实验步骤

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