Anti-Catalase/CAT Antibody

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of Catalase/CAT using anti-Catalase/CAT antibody (PB0971). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human Raji whole cell lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: rat PC-12 whole cell lysates,
  Lane 7: mouse liver tissue lysates,
  Lane 8: mouse 3T3-L1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Catalase/CAT antigA03957-Aen affinity purified polyclonal antibody (PB0971) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Catalase/CAT at approximately 60 kDa. The expected band size for Catalase/CAT is at 60 kDa.

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  IHC analysis of Catalase/CAT using anti-Catalase/CAT antibody (PB0971).
  Catalase/CAT was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Catalase/CAT Antibody (PB0971) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  ICC/IF analysis of Catalase/CAT using anti-Catalase/CAT antibody (PB0971).
  Catalase/CAT was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Catalase/CAT Antibody (PB0971) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of Jurkat cells using anti-Catalase/CAT antibody (PB0971).
  Overlay histogram showing Jurkat cells stained with PB0971 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Catalase/CAT Antibody (PB0971) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of Catalase/CAT using anti-Catalase/CAT antibody (PB0971). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human Raji whole cell lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: rat PC-12 whole cell lysates,
  Lane 7: mouse liver tissue lysates,
  Lane 8: mouse 3T3-L1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Catalase/CAT antigA03957-Aen affinity purified polyclonal antibody (PB0971) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Catalase/CAT at approximately 60 kDa. The expected band size for Catalase/CAT is at 60 kDa.

• 

  IHC analysis of Catalase/CAT using anti-Catalase/CAT antibody (PB0971).
  Catalase/CAT was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Catalase/CAT Antibody (PB0971) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

• 

  ICC/IF analysis of Catalase/CAT using anti-Catalase/CAT antibody (PB0971).
  Catalase/CAT was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Catalase/CAT Antibody (PB0971) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of Jurkat cells using anti-Catalase/CAT antibody (PB0971).
  Overlay histogram showing Jurkat cells stained with PB0971 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Catalase/CAT Antibody (PB0971) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.