Anti-HMGB2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ICC/IF FCM

PB10002

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HMGB2 using anti-HMGB2 antibody (PB10002). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB2 antigen affinity purified polyclonal antibody (PB10002) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB2 at approximately 24 kDa. The expected band size for HMGB2 is at 24 kDa.
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IHC analysis of HMGB2 using anti-HMGB2 antibody (PB10002).
HMGB2 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-HMGB2 Antibody (PB10002) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of HMGB2 using anti-HMGB2 antibody (PB10002).
HMGB2 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-HMGB2 Antibody (PB10002) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of HMGB2 using anti-HMGB2 antibody (PB10002).
HMGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-HMGB2 Antibody (PB10002) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of rat intestine tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).
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ICC/IF analysis of HMGB2 using anti-HMGB2 antibody (PB10002).
HMGB2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HMGB2 Antibody (PB10002) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
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ICC/IF analysis of HMGB2 using anti-HMGB2 antibody (PB10002) and anti-Alpha Tubulin antibody (M03989-3).
HMGB2 was detected in an immunocytochemical section of MCF7 cells. The section was incubated with rabbit anti-HMGB2 Antibody (PB10002) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
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Flow Cytometry analysis of A431 cells using anti-HMGB2 antibody (PB10002).
Overlay histogram showing A431 cells stained with PB10002 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGB2 Antibody (PB10002) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-HMGB2 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

high mobility group box 2; High mobility group protein 2; High mobility group protein B2; HMG 2; HMG2; HMGB2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, ICC/IF, FCM

基因名称

HMGB2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB2, identical to the related mouse and rat sequences.

计算分子量

24 kDa

实际分子量

24 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

High-mobility group protein B2, also known as high-mobility group protein 2 (HMG-2), is a protein that in humans is encoded by the HMGB2 gene. This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Uniprot ID

P26583

文献引用格式

HMGB2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB10002)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

high mobility group box 2

蛋白名全称

High mobility group protein B2

实验方案>

实验步骤

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