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Anti-MEF2A Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ICC/IF

PB1002

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of MEF2A using anti-MEF2A antibody (PB1002). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat thymus tissue lysates,
    Lane 2: human Hela whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MEF2A antigen affinity purified polyclonal antibody (PB1002) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MEF2A at approximately 55 kDa. The expected band size for MEF2A is at 55 kDa.

    all(6)
  • IHC analysis of MEF2A using anti-MEF2A antibody (PB1002).
    MEF2A was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MEF2A Antibody (PB1002) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of MEF2A using anti-MEF2A antibody (PB1002).
    MEF2A was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MEF2A Antibody (PB1002) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of MEF2A using anti-MEF2A antibody (PB1002).
    MEF2A was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MEF2A Antibody (PB1002) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of MEF2A using anti-MEF2A antibody (PB1002).
    MEF2A was detected in a paraffin-embedded section of human meningeoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MEF2A Antibody (PB1002) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • ICC/IF analysis of MEF2A using anti-MEF2A antibody (PB1002).
    MEF2A was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-MEF2A Antibody (PB1002) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(6)

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产品简介>

产品名称
Anti-MEF2A Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
ADCAD1; MEF2; MEF2A; myocyte enhancer factor 2A; RSRFC4; RSRFC9
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF
基因名称
MEF2A
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2A different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
计算分子量
55 kDa
实际分子量
55 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Myocyte-specific enhancer factor 2A is a protein that in humans is encoded by the MEF2A gene. It is mapped to 15q26. The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.
Uniprot ID
Q02078  
文献引用格式
MEF2A Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB1002)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
myocyte enhancer factor 2A
蛋白名全称
Myocyte-specific enhancer factor 2A
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-MEF2A Antibody

    筛选器: All WB IHC ICC/IF

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