Anti-AQP11 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB1098

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AQP11 using anti-AQP11 antibody (PB1098). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AQP11 antigen affinity purified polyclonal antibody (PB1098) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AQP11 at approximately 30 kDa/53 kDa. The expected band size for AQP11 is at 30 kDa.
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产品名称

Anti-AQP11 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AQP11; AQPX1; PSEC0027; Aquaporin-11; AQP-11

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

AQP11

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human AQP11 different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

计算分子量

30 kDa

实际分子量

30 kDa/53 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

AQP11 has a unique asparagine-proline-alanine (NPA) box distinct from those of other AQPs, suggesting a different pore structure and function. Using Northern blot analysis, they detected highest expression of mouse Aqp11 in testis, followed by liver and kidney. Expression was much weaker in heart, brain, and muscle. Western blot analysis of mouse kidney membrane fractions detected Aqp11 at an apparent molecular mass of 26 kD, lower than the calculated molecular mass of 30 kD. Immunohistochemical analysis localized Aqp11 to mouse renal proximal tubule cells, where it showed a perinuclear distribution. Fluorescence-tagged Aqp11 localized with an endoplasmic reticulum marker.

Uniprot ID

Q8NBQ7

文献引用格式

AQP11 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB1098)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aquaporin 11

蛋白名全称

Aquaporin-11

实验方案>

实验步骤

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