Anti-Ki67/MKI67 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ICC/IF

PB9026

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (PB9026). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Colo320 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human SKOV whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Ki67/MKI67 antigen affinity purified polyclonal antibody (PB9026) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Ki67/MKI67 at approximately 358 kDa. The expected band size for Ki67/MKI67 is at 358 kDa.
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Figure 2. IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (PB9026).
Ki67/MKI67 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-Ki67/MKI67 Antibody (PB9026) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Figure 3. IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (PB9026).
Ki67/MKI67 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-Ki67/MKI67 Antibody (PB9026) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Figure 4. IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (PB9026).
Ki67/MKI67 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Ki67/MKI67 Antibody (PB9026) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Figure 5. IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (PB9026).
Ki67/MKI67 was detected in a paraffin-embedded section of rat thymus tissue. The tissue section was incubated with rabbit anti-Ki67/MKI67 Antibody (PB9026) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
Figure 6. IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (PB9026).
Ki67/MKI67 was detected in a paraffin-embedded section of mouse thymus tissue. The tissue section was incubated with rabbit anti-Ki67/MKI67 Antibody (PB9026) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Figure 7. IF analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (PB9026).
Ki67/MKI67 was detected in a paraffin-embedded section of human colon organoid tissue. The tissue section was incubated with rabbit anti-Ki67/MKI67 Antibody (PB9026) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Figure 8. ICC analysis of Ki67/MKI67 using anti- Ki67/MKI67 antibody (PB9026).
Ki67/MKI67 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Ki67/MKI67 Antibody (PB9026) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Ki67/MKI67 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

D630048A14Rik; Ki 67; Ki67; Mki67

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, ICC/IF

基因名称

MKI67

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).

计算分子量

358 kDa

实际分子量

358 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Uniprot ID

PB9026

RRID

AB_3081930

文献引用格式

Ki67/MKI67 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9026)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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