Anti-ALOX15 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9073

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ALOX15 using anti-ALOX15 antibody (PB9073). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse testicular tissue lysates,
Lane 6: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALOX15 antigen affinity purified polyclonal antibody (PB9073) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALOX15 at approximately 75 kDa. The expected band size for ALOX15 is at 75 kDa.
all(5)
IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of Mouse Lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ALOX15 Antibody (PB9073) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ALOX15 Antibody (PB9073) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of Human Intestine Cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ALOX15 Antibody (PB9073) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ALOX15 Antibody (PB9073) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)

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产品简介>

产品名称

Anti-ALOX15 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

12/15-lipoxygenase; 12-LOX; Arachidonate 15-lipoxygenase; 15-LOX; 15-LOX-1; Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; ALOX15; LOG15; 12-LOX

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ALOX15

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.

计算分子量

75 kDa

实际分子量

75 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.

Uniprot ID

P16050

文献引用格式

ALOX15 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9073)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arachidonate 15-lipoxygenase

蛋白名全称

Arachidonate 15-lipoxygenase

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实验步骤

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