Anti-ALOX15 Antibody (Clone#OTI2G3)

Mouse monoclonal antibody

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筛选器： All WB IHC FCM

MA01222

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15.
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01222)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01222)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01222)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01222)
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALOX15 antibody, and then analyzed by flow cytometry.
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ALOX15 Antibody (Clone#OTI2G3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

12/15-lipoxygenase; 12-LOX; Arachidonate 15-lipoxygenase; 15-LOX; 15-LOX-1; Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; ALOX15; LOG15; 12-LOX

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, FCM

基因名称

ALOX15

克隆号

OTI2G3

宿主

Mouse

抗体亚型

IgG2b

免疫原

Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.

计算分子量

75 kDa

实际分子量

75 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.

Uniprot ID

P16050

文献引用格式

ALOX15 Antibody (Clone#OTI2G3) (Boster Biological Technology, Wuhan, China. Catalog#MA01222)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arachidonate 15-lipoxygenase

蛋白名全称

Arachidonate 15-lipoxygenase

实验方案>

实验步骤

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