Anti-ATF1 Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of ATF1 using anti-ATF1 antibody (PB9130). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A431 whole cell lysates,
  Lane 3: human SiHa whole cell lysates,
  Lane 4: rat testis tissue lysates,
  Lane 5: mouse testis tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF1 antigen affinity purified polyclonal antibody (PB9130) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF1 at approximately 35 kDa. The expected band size for ATF1 is at 29 kDa.

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  ICC/IF analysis of ATF1 using anti-ATF1 antibody (PB9130) and anti-Alpha Tubulin antibody (M03989-3).
  ATF1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ATF1 Antibody (PB9130) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.

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  Flow Cytometry analysis of PC-3 cells using anti-ATF1 antibody (PB9130).
  Overlay histogram showing PC-3 cells stained with PB9130 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF1 Antibody (PB9130) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of ATF1 using anti-ATF1 antibody (PB9130). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A431 whole cell lysates,
  Lane 3: human SiHa whole cell lysates,
  Lane 4: rat testis tissue lysates,
  Lane 5: mouse testis tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF1 antigen affinity purified polyclonal antibody (PB9130) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF1 at approximately 35 kDa. The expected band size for ATF1 is at 29 kDa.

• 

  ICC/IF analysis of ATF1 using anti-ATF1 antibody (PB9130) and anti-Alpha Tubulin antibody (M03989-3).
  ATF1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ATF1 Antibody (PB9130) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.

• 

  Flow Cytometry analysis of PC-3 cells using anti-ATF1 antibody (PB9130).
  Overlay histogram showing PC-3 cells stained with PB9130 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF1 Antibody (PB9130) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.