Western blot analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VWFpp/VWF antigen affinity purified polyclonal antibody (PB9273) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VWFpp/VWF at approximately 309 kDa. The expected band size for VWFpp/VWF is at 309 kDa.
Western blot analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse Lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VWFpp/VWF antigen affinity purified polyclonal antibody (PB9273) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VWFpp/VWF at approximately 309 kDa. The expected band size for VWFpp/VWF is at 309 kDa.
IHC analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273).
VWFpp/VWF was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VWFpp/VWF Antibody (PB9273) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273).
VWFpp/VWF was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VWFpp/VWF Antibody (PB9273) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- VWF antibody (PB9273). detected in paraffin-embedded section of rat liver tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(6) 
IF analysis using anti- VWF antibody (PB9273) and anti-alpha-Smooth Muscle Actin antibody (MA1106). detected in paraffin-embedded section of rat lung tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1031) and counterstained with DAPI (blue).
all(6) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VWFpp/VWF antigen affinity purified polyclonal antibody (PB9273) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VWFpp/VWF at approximately 309 kDa. The expected band size for VWFpp/VWF is at 309 kDa.
Western blot analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse Lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VWFpp/VWF antigen affinity purified polyclonal antibody (PB9273) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VWFpp/VWF at approximately 309 kDa. The expected band size for VWFpp/VWF is at 309 kDa.
IHC analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273).
VWFpp/VWF was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VWFpp/VWF Antibody (PB9273) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273).
VWFpp/VWF was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VWFpp/VWF Antibody (PB9273) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- VWF antibody (PB9273). detected in paraffin-embedded section of rat liver tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
IF analysis using anti- VWF antibody (PB9273) and anti-alpha-Smooth Muscle Actin antibody (MA1106). detected in paraffin-embedded section of rat lung tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1031) and counterstained with DAPI (blue).
Western blot analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VWFpp/VWF antigen affinity purified polyclonal antibody (PB9273) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VWFpp/VWF at approximately 309 kDa. The expected band size for VWFpp/VWF is at 309 kDa.
Western blot analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse Lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VWFpp/VWF antigen affinity purified polyclonal antibody (PB9273) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VWFpp/VWF at approximately 309 kDa. The expected band size for VWFpp/VWF is at 309 kDa.
IHC analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273).
VWFpp/VWF was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VWFpp/VWF Antibody (PB9273) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VWFpp/VWF using anti-VWFpp/VWF antibody (PB9273).
VWFpp/VWF was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VWFpp/VWF Antibody (PB9273) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- VWF antibody (PB9273). detected in paraffin-embedded section of rat liver tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
IF analysis using anti- VWF antibody (PB9273) and anti-alpha-Smooth Muscle Actin antibody (MA1106). detected in paraffin-embedded section of rat lung tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1031) and counterstained with DAPI (blue).
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