Western blot analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCT tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BSEP/ABCB11 antigen affinity purified polyclonal antibody (PB9414) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSEP/ABCB11 at approximately 146 kDa. The expected band size for BSEP/ABCB11 is at 146 kDa.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of ABCB11 using anti- ABCB11 antibody (PB9414)ABCB11 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- ABCB11 Antibody (PB9414) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
all(5) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCT tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BSEP/ABCB11 antigen affinity purified polyclonal antibody (PB9414) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSEP/ABCB11 at approximately 146 kDa. The expected band size for BSEP/ABCB11 is at 146 kDa.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of ABCB11 using anti- ABCB11 antibody (PB9414)ABCB11 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- ABCB11 Antibody (PB9414) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCT tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BSEP/ABCB11 antigen affinity purified polyclonal antibody (PB9414) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSEP/ABCB11 at approximately 146 kDa. The expected band size for BSEP/ABCB11 is at 146 kDa.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
BSEP/ABCB11 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of ABCB11 using anti- ABCB11 antibody (PB9414)ABCB11 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- ABCB11 Antibody (PB9414) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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