Anti-VDR Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9456

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of VDR using anti-VDR antibody (PB9456). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human Placenta tissue lysates,
Lane 2: Rat Kidney tissue lysates,
Lane 3: Rat Liver tissue lysates,
Lane 4: Rat Pancreas tissue lysates,
Lane 5: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VDR antigen affinity purified polyclonal antibody (PB9456) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VDR at approximately 48 kDa. The expected band size for VDR is at 48 kDa.
all(4)
IHC analysis of VDR using anti-VDR antibody (PB9456).
VDR was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VDR Antibody (PB9456) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of VDR using anti-VDR antibody (PB9456).
VDR was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VDR Antibody (PB9456) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of VDR using anti-VDR antibody (PB9456).
VDR was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VDR Antibody (PB9456) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-VDR Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

NR1I1; VDR; Vitamin D3 receptor

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

VDR

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human VDR, different from the related mouse and rat sequences by one amino acid.

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

VDR (Vitamin D Receptor), also known as Vitamin D Hormone Receptor, is a member of the nuclear receptor family of transcription factors. Labuda et al. (1991) assigned the VDR gene to 12q12-q14 by in situ hybridization. Using mutation analysis, Jurutka et al. (2000) characterized arg18/arg22, VDR residues immediately N-terminal of the first DNA-binding zinc finger, as vital for contact with the general transcription factor IIB (TFIIB). A natural polymorphic variant of VDR, termed F/M4 (missing a FokI restriction site), which lacks only the first 3 amino acids (including glu2), interacted more efficiently with TFIIB and also possessed elevated transcriptional activity compared with the full-length (f/M1) receptor. Shah et al. (2006) stated that the signaling and oncogenic activity of beta-catenin (CTNNB1) can be repressed by activation of VDR. Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25- dihydroxyvitamin D3.

Uniprot ID

P11473

RRID

AB_3082123

文献引用格式

VDR Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9456)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

vitamin D (1,25- dihydroxyvitamin D3) receptor

蛋白名全称

Vitamin D3 receptor

实验方案>

实验步骤

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