Anti-CD19 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF

PB9800

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD19 using anti-CD19 antibody (PB9800). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Ramos whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (PB9800) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD19 at approximately 95 kDa. The expected band size for CD19 is at 61 kDa.
all(3)
IHC analysis of CD19 using anti-CD19 antibody (PB9800) .
CD19 was detected in a paraffin-embedded section of human appendix tissue. The tissue section was incubated with rabbit anti-CD19 Antibody (PB9800) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IF analysis of CD19 using anti-CD19 antibody (PB9800).
CD19 was detected in a paraffin-embedded section of human tonsil cancer tissue. The tissue section was incubated with rabbit anti-CD19 Antibody (PB9800) at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)

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产品简介>

产品名称

Anti-CD19 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

B lymphocyte antigen CD19; B4; CD19; CD19 molecule; Differentiation antigen CD19; T cell surface antigen Leu 12

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, IF

基因名称

CD19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human CD19, different from the related mouse sequence by seven amino acids.

计算分子量

61 kDa

实际分子量

95 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Uniprot ID

P15391

RRID

AB_3082261

文献引用格式

CD19 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9800)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD19 molecule

蛋白名全称

B-lymphocyte antigen CD19

实验方案>

实验步骤

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