Anti-TCP1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PB9826

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TCP1 using anti-TCP1 antibody (PB9826). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human MOLT4 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human MCF-7 whole cell lysates,
Lane 8: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TCP1 antigen affinity purified polyclonal antibody (PB9826) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TCP1 at approximately 60 kDa. The expected band size for TCP1 is at 60 kDa.
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Western blot analysis of TCP1 using anti-TCP1 antibody (PB9826). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse ovary tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TCP1 antigen affinity purified polyclonal antibody (PB9826) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TCP1 at approximately 60 kDa. The expected band size for TCP1 is at 60 kDa.
all(7)
IHC analysis of TCP1 using anti-TCP1 antibody (PB9826) .
TCP1 was detected in a paraffin-embedded section of human ovary cancer tissue. The tissue section was incubated with rabbit anti-TCP1 Antibody (PB9826) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of TCP1 using anti-TCP1 antibody (PB9826) .
TCP1 was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-TCP1 Antibody (PB9826) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of TCP1 using anti-TCP1 antibody (PB9826) .
TCP1 was detected in a paraffin-embedded section of mouse testis tissue. The tissue section was incubated with rabbit anti-TCP1 Antibody (PB9826) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of TCP1 using anti-TCP1 antibody (PB9826) .
TCP1 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-TCP1 Antibody (PB9826) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IF analysis of TCP1 using anti-TCP1 antibody (PB9826).
TCP1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-TCP1 Antibody (PB9826) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(7)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-TCP1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CCT alpha; CCT1; CCTa; D6S230E; t complex 1; TCP 1 alpha; TCP1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

TCP1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TCP1, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

计算分子量

60 kDa

实际分子量

60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

T-complex protein 1 subunit alpha is a protein that in humans is encoded by the TCP1 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.

Uniprot ID

P17987

RRID

AB_3082266

文献引用格式

TCP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9826)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

t-complex 1

蛋白名全称

T-complex protein 1 subunit alpha

实验方案>

实验步骤

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