Anti-Cathepsin K/CTSK Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9856

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Cathepsin K/CTSK using anti-Cathepsin K/CTSK antibody (PB9856). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 22RV1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cathepsin K/CTSK antigen affinity purified polyclonal antibody (PB9856) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cathepsin K/CTSK at approximately 45 kDa. The expected band size for Cathepsin K/CTSK is at 37 kDa.
all(2)
IHC analysis of Cathepsin K/CTSK using anti-Cathepsin K/CTSK antibody (PB9856).
Cathepsin K/CTSK was detected in a paraffin-embedded section of human osteosarcoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cathepsin K/CTSK Antibody (PB9856) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-Cathepsin K/CTSK Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Cathepsin K; Cathepsin O; Cathepsin O2; Cathepsin X; catK; CTS02; CTSK; CTSO; CTSO1; CTSO2; PKND; PYCD

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

CTSK

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Cathepsin K recombinant protein (Position: A115-M329). Human Cathepsin K shares 86.9% and 88.8% amino acid (aa) sequence identity with mouse and rat Cathepsin K, respectively.

计算分子量

37 kDa

实际分子量

45 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cathepsin K, abbreviated°CTSK, is an enzyme that in humans is encoded by the°CTSK gene. It is mapped to 1q21. The protein encoded by this gene is a lysosomal°Cysteine protease involved in bone remodeling and resorption. And this protein, which is a member of the peptidase C1 protein family, is expressed predominantly in osteoclasts. Additionally, the enzyme's ability to catabolize elastin,°Collagen, and gelatin allow it to break down bone and cartilage. This catabolic activity is also partially responsible for the loss of lung elasticity and recoil inemphysema. Cathepsin K inhibitors, such as odanacatib, show great potential in the treatment of osteoporosis. Cathepsin K is degraded by Cathepsin S, called Controlled Cathepsin Cannibalism.

Uniprot ID

P43235

文献引用格式

Cathepsin K/CTSK Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9856)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cathepsin K

蛋白名全称

Cathepsin K

实验方案>

实验步骤

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