Anti-FOXP3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

A00011-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of FOXP3 using anti-FOXP3 antibody (A00011-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOXP3 antigen affinity purified polyclonal antibody (A00011-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FOXP3 at approximately 47 kDa. The expected band size for FOXP3 is at 47 kDa.
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产品名称

Anti-FOXP3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AIID; DIETER; forkhead box P3; Forkhead box protein P3; FOXP3; IPEX; JM2; PIDX; Scurfin; XPID

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

基因名称

FOXP3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of mouse Foxp3, which shares 94.4% amino acid (aa) sequence identity with human FOXP3.

计算分子量

47 kDa

实际分子量

47 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

FOXP3(forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome(specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.

Uniprot ID

Q99JB6

RRID

AB_3080878

文献引用格式

FOXP3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00011-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

forkhead box P3

蛋白名全称

Forkhead box protein P3

实验方案>

实验步骤

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