Anti-FOXP3 Antibody (Clone#OTI8G3)

Mouse monoclonal antibody

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筛选器： All FCM WB

MA00011

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Permeabilized HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXP3 antibody, and then analyzed by flow cytometry (1:100).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXP3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXP3 (1:2000).
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产品名称

Anti-FOXP3 Antibody (Clone#OTI8G3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AIID; DIETER; forkhead box P3; Forkhead box protein P3; FOXP3; IPEX; JM2; PIDX; Scurfin; XPID

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

FCM, WB

基因名称

FOXP3

克隆号

OTI8G3

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human FOXP3 (NP_001107849) produced in E.coli.

实际分子量

43.2 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

FOXP3(forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome(specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.

Uniprot ID

Q99JB6

文献引用格式

FOXP3 Antibody (Clone#OTI8G3) (Boster Biological Technology, Wuhan, China. Catalog#MA00011)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

forkhead box P3

蛋白名全称

Forkhead box protein P3

实验方案>

实验步骤

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