Anti-AKT1/2/3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF FCM ELISA

A00024-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AKT1/2/3 using anti-AKT1/2/3 antibody (A00024-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: Raji whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1/2/3 antigen affinity purified polyclonal antibody (A00024-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT1/2/3 at approximately 60 kDa. The expected band size for AKT1/2/3 is at 56 kDa.
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IF analysis of AKT1/2/3 using anti-AKT1/2/3 antibody (A00024-2).
AKT1/2/3 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-AKT1/2/3 Antibody (A00024-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
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Flow Cytometry analysis of Hela cells using anti-AKT1/2/3 antibody (A00024-2).
Overlay histogram showing Hela cells stained with A00024-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKT1/2/3 Antibody (A00024-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介>

产品名称

Anti-AKT1/2/3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AKT; AKT1; PKB; PKB ALPHA; PRKBA; Protein kinase B; Proto oncogene c Akt; RAC; RAC ALPHA; RAC PK alpha; AKT2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt 2; Protein kinase B beta; RAC BETA; RAC PK beta; AKT3; PKB GAMMA; PKBG; PRKBG; Protein kinase Akt 3; Protein kinase B gamma; RAC gamma; RAC PK gamma; STK 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, FCM, ELISA

基因名称

AKT1/AKT2/AKT3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human AKT1,2,3 recombinant protein (Position: E17-A477).

计算分子量

56 kDa

实际分子量

60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

Uniprot ID

P31749/P31751/Q9Y243

文献引用格式

AKT1/2/3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00024-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

AKT serine/threonine kinase 1,2,3

蛋白名全称

RAC-alpha/beta/gamma serine/threonine-protein kinase

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