Anti-AKT1/2/3 Antibody (Clone#17A20)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4400

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-AKT1/2/3 antibody (BM4400). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1/2/3 antigen affinity purified monoclonal antibody (BM4400) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT1/2/3 at approximately 60 kDa. The expected band size for AKT1/2/3 is at 60 kDa.
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Immunohistochemical analysis of paraffin-embedded human testis, using Akt(pan)1/2/3 Antibody.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody.
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Immunofluorescent analysis of MCF-7 cells, using Akt(pan)1/2/3 Antibody.
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Immunoprecipitate (IP) analysis using the Antibody. (wb)
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产品简介>

产品名称

Anti-AKT1/2/3 Antibody (Clone#17A20)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AKT; AKT1; PKB; PKB ALPHA; PRKBA; Protein kinase B; Proto oncogene c Akt; RAC; RAC ALPHA; RAC PK alpha; AKT2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt 2; Protein kinase B beta; RAC BETA; RAC PK beta; AKT3; PKB GAMMA; PKBG; PRKBG; Protein kinase Akt 3; Protein kinase B gamma; RAC gamma; RAC PK gamma; STK 2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

AKT1/AKT2/AKT3

克隆号

17A20

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human AKT1/2/3

计算分子量

60 kDa

实际分子量

60 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.

背景资料

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1.

Uniprot ID

P31749/P31751/Q9Y243

文献引用格式

AKT1/2/3 Antibody (Clone#17A20) (Boster Biological Technology, Wuhan, China. Catalog#BM4400)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

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