Anti-CXCR4 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human U87 whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human MCF-7 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: rat PC-12 whole cell lysates,
  Lane 7: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (A00031-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CXCR4 at approximately 40-50 kDa. The expected band size for CXCR4 is at 40 kDa.

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  Flow Cytometry analysis of THP-1 cells using anti-CXCR4 antibody (A00031-4).
  Overlay histogram showing THP-1 cells stained with A00031-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR4 Antibody (A00031-4) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human U87 whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human MCF-7 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: rat PC-12 whole cell lysates,
  Lane 7: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (A00031-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CXCR4 at approximately 40-50 kDa. The expected band size for CXCR4 is at 40 kDa.

• 

  Flow Cytometry analysis of THP-1 cells using anti-CXCR4 antibody (A00031-4).
  Overlay histogram showing THP-1 cells stained with A00031-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR4 Antibody (A00031-4) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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