Anti-CXCR4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PA1237

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CXCR4 using anti-CXCR4 antibody (PA1237). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (PA1237) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CXCR4 at approximately 40-60 kDa. The expected band size for CXCR4 is at 40 kDa.
all(2)
IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237).
CXCR4 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CXCR4 Antibody (PA1237) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-CXCR4 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD184; CXC R4; CXCR 4; CXCR4; CXCR4B; D2S201E; FB22; Fusin; HM89; HSY3RR; LAP3; LCR1; LESTR; SDF 1 receptor; WHIM

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

CXCR4

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4, different from the related rat and mouse sequences by two amino acids.

计算分子量

40 kDa

实际分子量

40-60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CXCR4 (Chemokine,CXC Motif, Receptor 4), also known as FUSIN or NPY3R, is a protein that in humans is encoded by the CXCR4 gene. It is the receptor for the CXC chemokine SDF1 that has essential functions on embryo organogenesis, immunological functions and T lymphocyte trafficking. CXCR4 is the only SDF1 receptor identified so far. This suggests that CXCR4 expression is critical for the biological effects of SDF1. CXCR4 is also a seven-transmembrane-spanning, G-protein-coupled receptor for the CXC chemokine PBSF/SDF-1. It functions as a co-receptor for T-cell-line tropic human immunodeficiency virus HIV-1. It was concluded that PBSF/SDF-1 and CXCR4 define a new signalling system for organ vascularization.

Uniprot ID

P61073

RRID

AB_3082606

文献引用格式

CXCR4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA1237)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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