Anti-TAU/MAPT Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF ELISA

A00097-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TAU/MAPT using anti-TAU/MAPT antibody (A00097-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TAU/MAPT antigen affinity purified polyclonal antibody (A00097-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TAU/MAPT at approximately 50-80 kDa. The expected band size for TAU/MAPT is at 79 kDa.
all(3)
IF analysis of TAU/MAPT using anti-TAU/MAPT antibody (A00097-3).
TAU/MAPT was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-TAU/MAPT Antibody (A00097-3) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)
all(3)

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产品简介>

产品名称

Anti-TAU/MAPT Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

DDPAC; FTDP 17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament tau; PHF tau; PPND; TAU

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, ELISA

基因名称

MAPT

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Tau/MAPT recombinant protein (Position: M1-L322).

计算分子量

79 kDa

实际分子量

50-80 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

MAPT, Microtubule-associated protein tau, appears to be enriched in axons. The MAPT gene is assigned to chromosome 17 by hybridization of a cDNA clone to flow-sorted and spot-blotted chromosomes and to 17q21 by in situ hybridization, containing 16 exons. The tau proteins are the product of alternative splicingfrom a single gene that in humans is designated MAPT. Tau proteins are proteins that stabilize microtubules. They are abundant in neurons in the central nervous system and are less common elsewhere. When tau proteins are defective, and no longer stabilize microtubules properly, they can result in dementias such as Alzheimer's disease.

Uniprot ID

P10636

文献引用格式

TAU/MAPT Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00097-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

microtubule associated protein tau

蛋白名全称

Microtubule-associated protein tau

实验方案>

实验步骤

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