Anti-TAU/MAPT (Phospho-T231) Antibody (Clone#EIH-13)

Rabbit Monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC IP

BM4464

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-TAU/MAPT antibody (BM4464). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TAU/MAPT antigen affinity purified monoclonal antibody (BM4464) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TAU/MAPT at approximately 50-70 kDa. The expected band size for TAU/MAPT is at 79 kDa.
all(6)
All lanes use the Antibody for 1 hour at room temperature.
all(6)
Western blot analysis of Phospho-Tau (T231) expression in (1) SH-SY5Y cell lysate; (2) SH-SY5Y cell lysate, treated with sorbitol.
all(6)
IHC analysis of TAU/MAPT using anti-TAU/MAPT antibody (BM4464) .
TAU/MAPT was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-TAU/MAPT Antibody (BM4464) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of TAU/MAPT using anti-TAU/MAPT antibody (BM4464) .
TAU/MAPT was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-TAU/MAPT Antibody (BM4464) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-TAU/MAPT (Phospho-T231) Antibody (Clone#EIH-13)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

DDPAC; FTDP 17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament tau; PHF tau; PPND; TAU

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

MAPT

克隆号

EIH-13

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-Tau (T231)

计算分子量

79 kDa

实际分子量

50-80 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5. Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies.

Uniprot ID

P10636

文献引用格式

TAU/MAPT (Phospho-T231) Antibody (Clone#EIH-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4464)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Microtubule-associated protein tau

实验方案>

实验步骤

引用文献(0)>

清除