规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
major histocompatibility complex; class I; A; HLAA; HLA-A
应用范围
WB, IHC, ICC/IF, IP, FCM, ELISA
免疫原
E.coli-derived human HLA-A recombinant protein (Position: S26-T305).
成分
500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
保存条件
12 months from date of receipt，-20℃ as supplied.
背景资料
HLA-A, namely HLA A, is a group of human leukocyte antigens (HLA) that are coded for by the HLA-A locus, which is located at human chromosome 6p21.3. HLA is simply the major histocompatibility complex (MHC) specific to humans. HLA-A is one of three major types of human MHC class I cell surface receptors. The others are HLA-B and HLA-C. And it is critical to the cytotoxic t-cell controlled immune response to viruses and other intracellular pathogens. Because each HLA-A gene has a high affinity for slightly different peptides, certain HLA-As are associated with increased risk, more rapid progression, and/or increased severity of many diseases. For similar reasons, HLA-A matching is essential to successful tissue transplants.
文献引用格式
HLA-A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00194-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
基因名全称
major histocompatibility complex, class I, A
蛋白名全称
HLA class I histocompatibility antigen, A-2 alpha chain
推荐稀释比
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| ImmunoPrecipitation (IP): | 1:50 |
| Flow Cytometry (FCM): | 1-3 μg/1x106 cells |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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