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Anti-HLA-A Antibody (Clone#BBI-8)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF IP FCM

BM4084

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-HLA-A antibody (BM4084). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human A549 whole cell lysates,
    Lane 2: human THP-1 whole cell lysates,
    Lane 3: human Jurkat whole cell lysates,
    Lane 4: human Raji whole cell lysates,
    Lane 5: human Daudi whole cell lysates,
    Lane 6: human A431 whole cell lysates,
    Lane 7: human HEL whole cell lysates,
    Lane 8: human RT4 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HLA-A antigen affinity purified monoclonal antibody (BM4084) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HLA-A at approximately 41 kDa. The expected band size for HLA-A is at 41 kDa.

    all(5)
  • IHC analysis of HLA-A using anti-HLA-A antibody (BM4084) .
    HLA-A was detected in a paraffin-embedded section of human pancreas cancer tissue. The tissue section was incubated with rabbit anti-HLA-A Antibody (BM4084) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(5)

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产品名称
Anti-HLA-A Antibody (Clone#BBI-8)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
major histocompatibility complex; class I; A; HLAA; HLA-A
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF, IP, FCM
基因名称
HLA-A
克隆号
BBI-8
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human MHC class 1
计算分子量
41 kDa
实际分子量
41 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
HLA-A, namely HLA A, is a group of human leukocyte antigens (HLA) that are coded for by the HLA-A locus, which is located at human chromosome 6p21.3. HLA is simply the major histocompatibility complex (MHC) specific to humans. HLA-A is one of three major types of human MHC class I cell surface receptors. The others are HLA-B and HLA-C. And it is critical to the cytotoxic t-cell controlled immune response to viruses and other intracellular pathogens. Because each HLA-A gene has a high affinity for slightly different peptides, certain HLA-As are associated with increased risk, more rapid progression, and/or increased severity of many diseases. For similar reasons, HLA-A matching is essential to successful tissue transplants.
Uniprot ID
P01891  
文献引用格式
HLA-A Antibody (Clone#BBI-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4084)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
HLA class I histocompatibility antigen, A-68 alpha chain
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:20
Flow Cytometry (FCM):1:20

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    Anti-HLA-A Antibody (Clone#BBI-8)

    筛选器: All WB IHC ICC/IF IP FCM

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