| Western blot (WB): | 1:500-1000 |

Western blot analysis of extracts (35ug) from RPMI-8226 lysate by using anti-HLA-A monoclonal antibody. (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLA-A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLA-A.(1:1000)

Western blot analysis of extracts (35ug) from CCRF-CEM lysate by using anti-HLA-A monoclonal antibody. (1:500)

Western blot analysis of extracts (35ug) from RPMI-8226 lysate by using anti-HLA-A monoclonal antibody. (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLA-A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLA-A.(1:1000)

Western blot analysis of extracts (35ug) from CCRF-CEM lysate by using anti-HLA-A monoclonal antibody. (1:500)


