Anti-ARID1A Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM ELISA

A00247-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ARID1A using anti-ARID1A antibody (A00247-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID1A antigen affinity purified polyclonal antibody (A00247-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID1A at approximately 250-270 kDa. The expected band size for ARID1A is at 242 kDa.
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IHC analysis of ARID1A using anti-ARID1A antibody (A00247-2) .
ARID1A was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody (A00247-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ARID1A using anti-ARID1A antibody (A00247-2) .
ARID1A was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody (A00247-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ARID1A using anti-ARID1A antibody (A00247-2) .
ARID1A was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody (A00247-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ARID1A using anti-ARID1A antibody (A00247-2) .
ARID1A was detected in a paraffin-embedded section of rat thymus tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody (A00247-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of ARID1A using anti-ARID1A antibody (A00247-2) and anti-Beta Tubulin antibody (M01857-3).
ARID1A was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ARID1A Antibody (A00247-2) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
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Flow Cytometry analysis of K562 cells using anti-ARID1A antibody (A00247-2).
Overlay histogram showing K562 cells stained with A00247-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID1A Antibody (A00247-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ARID1A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ARID1A; B120; BAF250; BAF250a; BM029; BRG1 associated factor 250; BRG1 associated factor 250a; C1orf4; hELD; hOSA1; Osa homolog 1; OSA1; P270; SMARCF1; SWI like protein; SWI/SNF complex protein p270

产品类型

Polyclonal

检验物种

human, rat

应用范围

WB, IHC, ICC/IF, FCM, ELISA

基因名称

ARID1A

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ARID1A recombinant protein (Position: R857-Q1552).

计算分子量

242 kDa

实际分子量

250-270 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

AT-rich interactive domain-containing protein 1A is a protein that in humans is encoded by the ARID1A gene. This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

O14497

RRID

AB_3081026

文献引用格式

ARID1A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00247-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

AT-rich interaction domain 1A

蛋白名全称

AT-rich interactive domain-containing protein 1A

实验方案>

实验步骤

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