Anti-ARID1A Antibody (Clone#17A27)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4822

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ARID1A antibody (BM4822). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A375 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID1A antigen affinity purified monoclonal antibody (BM4822) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID1A at approximately 270 kDa. The expected band size for ARID1A is at 242 kDa.
all(3)
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using ARID1A Antibody.
all(3)
Immunofluorescent analysis of SH-SY5Y cells, using ARID1A Antibody.
all(3)

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产品简介>

产品名称

Anti-ARID1A Antibody (Clone#17A27)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ARID1A; B120; BAF250; BAF250a; BM029; BRG1 associated factor 250; BRG1 associated factor 250a; C1orf4; hELD; hOSA1; Osa homolog 1; OSA1; P270; SMARCF1; SWI like protein; SWI/SNF complex protein p270

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

ARID1A

克隆号

17A27

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ARID1A

计算分子量

242 kDa

实际分子量

250-270 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

Uniprot ID

O14497

文献引用格式

ARID1A Antibody (Clone#17A27) (Boster Biological Technology, Wuhan, China. Catalog#BM4822)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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