Anti-ARID1A Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of ARID1A using anti-ARID1A antibody (PB0724). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human K562 whole cell lysates,
  Lane 2: human THP-1 whole cell lysates,
  Lane 3: human Jurkat whole cell lysates,
  Lane 4: human SH-SY5Y whole cell lysates,
  Lane 5: mouse testis tissue lysates,
  Lane 6: mouse thymus tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID1A antigA03957-Aen affinity purified polyclonal antibody (PB0724) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID1A at approximately 250-270 kDa. The expected band size for ARID1A is at 242 kDa.

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  IF analysis of ARID1A using anti-ARID1A antibody (PB0724).
  ARID1A was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ARID1A Antibody (PB0724) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of Hela cells using anti-ARID1A antibody (PB0724).
  Overlay histogram showing Hela cells stained with PB0724 (Blue line). To facilitate intrARID1Allular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID1A Antibody (PB0724) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of ARID1A using anti-ARID1A antibody (PB0724). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human K562 whole cell lysates,
  Lane 2: human THP-1 whole cell lysates,
  Lane 3: human Jurkat whole cell lysates,
  Lane 4: human SH-SY5Y whole cell lysates,
  Lane 5: mouse testis tissue lysates,
  Lane 6: mouse thymus tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID1A antigA03957-Aen affinity purified polyclonal antibody (PB0724) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ARID1A at approximately 250-270 kDa. The expected band size for ARID1A is at 242 kDa.

• 

  IF analysis of ARID1A using anti-ARID1A antibody (PB0724).
  ARID1A was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ARID1A Antibody (PB0724) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of Hela cells using anti-ARID1A antibody (PB0724).
  Overlay histogram showing Hela cells stained with PB0724 (Blue line). To facilitate intrARID1Allular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID1A Antibody (PB0724) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.