Western blot analysis of anti-CD326/Epcam antibody (A00276-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/Epcam antigen affinity purified polyclonal antibody (A00276-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/Epcam at approximately 38 kDa. The expected band size for CD326/Epcam is at 35,40 kDa.
IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of rat colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)(Catalog#BA1105) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of rat colon tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)(Catalog#BA1105) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of RAW264.7 cells using anti-CD326/Epcam antibody (A00276-2).
Overlay histogram showing RAWA264.7 cells stained with A00276-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD326/Epcam Antibody (A00276-2, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti-CD326/Epcam antibody (A00276-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/Epcam antigen affinity purified polyclonal antibody (A00276-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/Epcam at approximately 38 kDa. The expected band size for CD326/Epcam is at 35,40 kDa.
IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of rat colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)(Catalog#BA1105) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of rat colon tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)(Catalog#BA1105) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of RAW264.7 cells using anti-CD326/Epcam antibody (A00276-2).
Overlay histogram showing RAWA264.7 cells stained with A00276-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD326/Epcam Antibody (A00276-2, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of anti-CD326/Epcam antibody (A00276-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/Epcam antigen affinity purified polyclonal antibody (A00276-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/Epcam at approximately 38 kDa. The expected band size for CD326/Epcam is at 35,40 kDa.
IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of rat colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)(Catalog#BA1105) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2).
CD326/Epcam was detected in a paraffin-embedded section of rat colon tissue. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)(Catalog#BA1105) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of RAW264.7 cells using anti-CD326/Epcam antibody (A00276-2).
Overlay histogram showing RAWA264.7 cells stained with A00276-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD326/Epcam Antibody (A00276-2, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
